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Product Info Summary
| SKU: | A03546 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-ATG14 Antibody
SKU/Catalog Number
A03546
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ATG14 Antibody (Catalog # A03546). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ATG14 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ATG14 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03546)
Host
Rabbit
Contents
ATG14 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
ATG14 antibody was raised against an 18 amino acid synthetic peptide near the center of human ATG14. The immunogen is located within amino acids 270 - 320 of ATG14.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of ATG14 are known to exist; this antibody will detect all three isoforms
Reactive Species
A03546 is reactive to ATG14 in Human, Mouse, Rat
Applications
A03546 is guaranteed for ELISA, IF, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
55.309kDa
Background of ATG14
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG14 is a subunit of a class III phosphatidylinositol (PtdIns) 3-kinase complex that targets the complex to the endoplasmic reticulum, the site of autophagosome formation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ATG14 antibody can be used for detection of ATG14 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ATG14 in human small intestine tissue lysate with ATG14 antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of ATG14 in human small intestine tissue with ATG14 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ATG14 (Source: Uniprot.org, NCBI)
Gene Name
ATG14
Full Name
Beclin 1-associated autophagy-related key regulator
Weight
55.309kDa
Superfamily
ATG14 family
Alternative Names
ATG14 Autophagy Related 14 Homolog (S. Cerevisiae); ATG14 Autophagy Related 14 Homolog; ATG14L; Autophagy Related 14; Autophagy-Related Protein 14-Like Protein; Barkor; Beclin 1-Associated Autophagy-Related Key Regulator; Beclin 1-interacting protein; KIAA0831 ATG14 ATG14L, BARKOR, KIAA0831 autophagy related 14 beclin 1-associated autophagy-related key regulator|ATG14 autophagy related 14 homolog|Beclin 1-Interacting protein|autophagy-related protein 14-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATG14, check out the ATG14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATG14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATG14 Antibody (A03546)
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No publications found for A03546
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Customer Reviews
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